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resource source identifier antibodies gst tag monoclonal antibody proteintech rrid ab 2881488  (Proteintech)


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    Proteintech resource source identifier antibodies gst tag monoclonal antibody proteintech rrid ab 2881488
    Resource Source Identifier Antibodies Gst Tag Monoclonal Antibody Proteintech Rrid Ab 2881488, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gst+proteintech/GST+Tag/pm41831230-244-2-10
    Average 94 stars, based on 66 article reviews
    resource source identifier antibodies gst tag monoclonal antibody proteintech rrid ab 2881488 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Western Blot:

    Article Title: Ubiquitin like protein FAT10 repressed cardiac fibrosis after myocardial ischemic via mediating degradation of Smad3 dependent on FAT10-proteasome system
    Article Snippet: .. The following antibodies were used for western blotting and immunostaining Target protein Source (Catalog number) FAT10 Abcam (ab168680) Smad3 Abcam (ab40854) Smad3 66516-1-Ig UBD Fushon Protein Proteintech (Ag27706) Recombinant Human Smad3 protein Abcam (ab89353) Smooth Muscle Actin Proteintech (14395-1-AP) Collagen Type I Proteintech (14695-1-AP) Collagen Type III Proteintech (22734-1-AP) p-Smad3 Abcam (ab52903) Smad2 Abcam (ab40855) p-Smad2 Abcam (ab280888) Smad4 Abcam (EP618Y) Smad7 Proteintech (25840-1-AP) GST Proteintech (10000-0-AP) Flag Proteintech (66008-2-Ig) HA Proteintech (51064-2-AP) His Proteintech (66005-1-Ig) GAPDH Proteintech (60004-1-Ig) MG132 Selleck (S2619) CHX Selleck (S3648) TNF-α/IFN-γ PeproTech Supplemental Figures Supplemental Figure S1. ..

    Immunostaining:

    Article Title: Ubiquitin like protein FAT10 repressed cardiac fibrosis after myocardial ischemic via mediating degradation of Smad3 dependent on FAT10-proteasome system
    Article Snippet: .. The following antibodies were used for western blotting and immunostaining Target protein Source (Catalog number) FAT10 Abcam (ab168680) Smad3 Abcam (ab40854) Smad3 66516-1-Ig UBD Fushon Protein Proteintech (Ag27706) Recombinant Human Smad3 protein Abcam (ab89353) Smooth Muscle Actin Proteintech (14395-1-AP) Collagen Type I Proteintech (14695-1-AP) Collagen Type III Proteintech (22734-1-AP) p-Smad3 Abcam (ab52903) Smad2 Abcam (ab40855) p-Smad2 Abcam (ab280888) Smad4 Abcam (EP618Y) Smad7 Proteintech (25840-1-AP) GST Proteintech (10000-0-AP) Flag Proteintech (66008-2-Ig) HA Proteintech (51064-2-AP) His Proteintech (66005-1-Ig) GAPDH Proteintech (60004-1-Ig) MG132 Selleck (S2619) CHX Selleck (S3648) TNF-α/IFN-γ PeproTech Supplemental Figures Supplemental Figure S1. ..

    Ubiquitin Proteomics:

    Article Title: Ubiquitin like protein FAT10 repressed cardiac fibrosis after myocardial ischemic via mediating degradation of Smad3 dependent on FAT10-proteasome system
    Article Snippet: .. The following antibodies were used for western blotting and immunostaining Target protein Source (Catalog number) FAT10 Abcam (ab168680) Smad3 Abcam (ab40854) Smad3 66516-1-Ig UBD Fushon Protein Proteintech (Ag27706) Recombinant Human Smad3 protein Abcam (ab89353) Smooth Muscle Actin Proteintech (14395-1-AP) Collagen Type I Proteintech (14695-1-AP) Collagen Type III Proteintech (22734-1-AP) p-Smad3 Abcam (ab52903) Smad2 Abcam (ab40855) p-Smad2 Abcam (ab280888) Smad4 Abcam (EP618Y) Smad7 Proteintech (25840-1-AP) GST Proteintech (10000-0-AP) Flag Proteintech (66008-2-Ig) HA Proteintech (51064-2-AP) His Proteintech (66005-1-Ig) GAPDH Proteintech (60004-1-Ig) MG132 Selleck (S2619) CHX Selleck (S3648) TNF-α/IFN-γ PeproTech Supplemental Figures Supplemental Figure S1. ..

    Recombinant:

    Article Title: Ubiquitin like protein FAT10 repressed cardiac fibrosis after myocardial ischemic via mediating degradation of Smad3 dependent on FAT10-proteasome system
    Article Snippet: .. The following antibodies were used for western blotting and immunostaining Target protein Source (Catalog number) FAT10 Abcam (ab168680) Smad3 Abcam (ab40854) Smad3 66516-1-Ig UBD Fushon Protein Proteintech (Ag27706) Recombinant Human Smad3 protein Abcam (ab89353) Smooth Muscle Actin Proteintech (14395-1-AP) Collagen Type I Proteintech (14695-1-AP) Collagen Type III Proteintech (22734-1-AP) p-Smad3 Abcam (ab52903) Smad2 Abcam (ab40855) p-Smad2 Abcam (ab280888) Smad4 Abcam (EP618Y) Smad7 Proteintech (25840-1-AP) GST Proteintech (10000-0-AP) Flag Proteintech (66008-2-Ig) HA Proteintech (51064-2-AP) His Proteintech (66005-1-Ig) GAPDH Proteintech (60004-1-Ig) MG132 Selleck (S2619) CHX Selleck (S3648) TNF-α/IFN-γ PeproTech Supplemental Figures Supplemental Figure S1. ..

    Article Title: Nuclear Import Receptors Directly Bind to Arginine-Rich Dipeptide Repeat Proteins and Suppress Their Pathological Interactions
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti Importin b (Frohnert et al., 2014), gift from Ralph Kehlenbach, University of Goettingen N/A Mouse anti TNPO1 clone D45 Sigma Cat# T0825, RRID:AB_262123 Goat anti CRM1 (Roloff et al., 2013), gift from Ralph Kehlenbach, University of Goettingen N/A Rabbit anti TDP-43 Proteintech Cat# 10782-2-AP RRID:AB_615042 Mouse anti TDP-43 Proteintech Cat# 60019-2-Ig RRID:AB_2200520 Goat anti TIA-1 Santa Cruz Cat# sc-48371 RRID:AB_628358 Rabbit anti GST Proteintech Cat# 10000-0-AP RRID:AB_1104231 Mouse anti His6 Abcam Cat# ab18184 RRID:AB_444306 Alexa 647 Donkey anti-goat Thermo Cat# A-21447 RRID:AB_141844 IRDye 680RD Donkey anti-Mouse IgG LI-COR Cat# P/N 926-68072 RRID:AB_10953628 IRDye 800RD Donkey anti-Rabbit IgG LI-COR Cat# P/N: 926-32213 RRID:AB_62184 Chemicals, Peptides, and Recombinant Proteins GST-GR25 This paper N/A TMR-GR10 Pepscan N/A TMR-GR25 Genscript; Pepscan N/A TMR-PR25 Genscript; Pepscan N/A TMR-GP25 Pepscan N/A .. Biotin-GR25 PSL N/A Biotin-PR25 PSL N/A Biotin-GP25 PSL N/A GR20-FLAG DGpeptides N/A His6-Tev-TNPO1 (Suárez-Calvet et al., 2016) N/A GST-Tev-TNPO1 (Lee et al., 2016) N/A His6-S-Impb (Chi et al., 1997) N/A His6-Impa1 (Weis et al., 1995), gift from Angus Lamond, University of Dundee N/A His6-Impa3 This paper N/A His6-TNPO3 (Bourgeois et al., 2020) N/A His6-TEV (Hofweber et al., 2018) N/A CRM1 (Askjaer et al., 1999; G€uttler et al., 2010) N/A TDP-43-Tev-MBP-His6 (Wang et al., 2018) N/A (Continued on next page) e1 Cell Reports 33, 108538, December 22, 2020

    Hemagglutination Assay:

    Article Title: DDX6 interacts with DDX3X to repress translation in microRNA-mediated silencing
    Article Snippet: .. CXCR miRNA: TOP: 5’-GUU UUC ACA AAG CUA ACA CA-3’ CXCR miRNA BOTTOM: 5’-UGU UAG CUG GAG UGA AAA CUU-3’ mir-30 TOP: GCU GUA AAC AUC CUC GAC UGG AAG UU mir-30 BOTTOM: CUU UCA GUC GGA UGU UUG CAG CUU Table S3: Antibodies used in this paper DDX6 Bethyl A310-131A, rabbit anti-DDX6, 1:5000, MW 55 KDa Bethyl A310-460A, rabbit anti-DDX6, 1:5000 DDX3X Bethyl A300-474A, rabbit anti-DDX3, 1:5000, MW 75kDa Flag Sigma F3165, Anti-Flag M2 Monoclonal Antibody, 1:2000 HA Sigma H6908, rabbit anti-HA antibody, 1:2000 Roche Appplied Science, 11 583 816 001, Mouse, 1:2000 V5 AbD Serotec Clone SV5-PK1 mouse, 1:2000 Myc DSHB, 9E10 mouse antibody 1:20 GFP Invitrogen, A-11122, rabbit antibody, 1:1000 Bim Enzo Life Sciences, ADI-AAP-330, rabbit anti-Bim/BOD polyclonal Ab, 1:1000, MW 23, 16, 13 kDa Bim Cell Signaling 2933, rabbit mAb (C34C5), 1;1000, MW 23, 16, 13 kDa 4E-T Fortis, A300-706A, rabbit anti-4E-T/eIF4E-T antibody affinity purified, 1:1000, 110 KDa His Novagen 70796-4, His Tag Monoclonal antibody, 1:4000 GST Proteintech, 66001-1-lg, Mouse antibody, 1:50000, MW 26kDa Caspase 2 Cell Signaling #2224, mouse antibody, 1:1000, MW 12, 14, 48kDa GAPDH GAPDH (6C5) antibody Santa Cruz Biotechnology Cat# sc-32233, RRID:AB_627679 Dcp2 Bethyl A302-597A, Rabbit anti-Dcp2, 1:2000, MW about 55kDa Puromycin Mouse Anti-Puromycin antibody (3RH11), KeraFAST, 1:2000 eIF3A Cell Signaling 3411, anti-eIF3A (D51F4) XP TM Rabbit mAb, 1:1000, MW 166 kDa RPL7 Bethyl, A300-740A, MW 29kDa Tubulin Beta III isoform Millipore MAB1637, Mouse, 1:2000, MW 55 kDa Flag Anti-FLAG M2 Affinity Gel, Sigma, A2220 Total protein quantification LI-COR P/N 926-11010, Revert 700 Total protein Stain Kit HRP-conjugated secondary antibody Goat anti-Mouse IgG(H/L):HRP, BioRad 5178-2504, 1:100-1:5000 Goat anti-rabbit IgG(H/L):HRP, BioRad, 5196-2504 , 1:1000-1:5000 DyLight 800 secondary antibody Invitrogen SA5-35571, goat anti-Rabbit IgG DyLight 800 FITC- or Cy3 conjugated secondary antibody CyTM3-conjugated AffiniPure Donkey Anti-Rabbit IgG (H+L), Jackson ImmunoResearch, 711-165-152, 1:200 Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L), Jackson ImmunoResearch 115-545-166, 1:200 Alexa Fluor 488-conjugated AffiniPure Donkey Anti-Rabbit IgG (H+L), Jackson ImmunoResearch 711-545-152, 1:200 Figure S1. ..

    Cell-Signaling:

    Article Title: DDX6 interacts with DDX3X to repress translation in microRNA-mediated silencing
    Article Snippet: .. CXCR miRNA: TOP: 5’-GUU UUC ACA AAG CUA ACA CA-3’ CXCR miRNA BOTTOM: 5’-UGU UAG CUG GAG UGA AAA CUU-3’ mir-30 TOP: GCU GUA AAC AUC CUC GAC UGG AAG UU mir-30 BOTTOM: CUU UCA GUC GGA UGU UUG CAG CUU Table S3: Antibodies used in this paper DDX6 Bethyl A310-131A, rabbit anti-DDX6, 1:5000, MW 55 KDa Bethyl A310-460A, rabbit anti-DDX6, 1:5000 DDX3X Bethyl A300-474A, rabbit anti-DDX3, 1:5000, MW 75kDa Flag Sigma F3165, Anti-Flag M2 Monoclonal Antibody, 1:2000 HA Sigma H6908, rabbit anti-HA antibody, 1:2000 Roche Appplied Science, 11 583 816 001, Mouse, 1:2000 V5 AbD Serotec Clone SV5-PK1 mouse, 1:2000 Myc DSHB, 9E10 mouse antibody 1:20 GFP Invitrogen, A-11122, rabbit antibody, 1:1000 Bim Enzo Life Sciences, ADI-AAP-330, rabbit anti-Bim/BOD polyclonal Ab, 1:1000, MW 23, 16, 13 kDa Bim Cell Signaling 2933, rabbit mAb (C34C5), 1;1000, MW 23, 16, 13 kDa 4E-T Fortis, A300-706A, rabbit anti-4E-T/eIF4E-T antibody affinity purified, 1:1000, 110 KDa His Novagen 70796-4, His Tag Monoclonal antibody, 1:4000 GST Proteintech, 66001-1-lg, Mouse antibody, 1:50000, MW 26kDa Caspase 2 Cell Signaling #2224, mouse antibody, 1:1000, MW 12, 14, 48kDa GAPDH GAPDH (6C5) antibody Santa Cruz Biotechnology Cat# sc-32233, RRID:AB_627679 Dcp2 Bethyl A302-597A, Rabbit anti-Dcp2, 1:2000, MW about 55kDa Puromycin Mouse Anti-Puromycin antibody (3RH11), KeraFAST, 1:2000 eIF3A Cell Signaling 3411, anti-eIF3A (D51F4) XP TM Rabbit mAb, 1:1000, MW 166 kDa RPL7 Bethyl, A300-740A, MW 29kDa Tubulin Beta III isoform Millipore MAB1637, Mouse, 1:2000, MW 55 kDa Flag Anti-FLAG M2 Affinity Gel, Sigma, A2220 Total protein quantification LI-COR P/N 926-11010, Revert 700 Total protein Stain Kit HRP-conjugated secondary antibody Goat anti-Mouse IgG(H/L):HRP, BioRad 5178-2504, 1:100-1:5000 Goat anti-rabbit IgG(H/L):HRP, BioRad, 5196-2504 , 1:1000-1:5000 DyLight 800 secondary antibody Invitrogen SA5-35571, goat anti-Rabbit IgG DyLight 800 FITC- or Cy3 conjugated secondary antibody CyTM3-conjugated AffiniPure Donkey Anti-Rabbit IgG (H+L), Jackson ImmunoResearch, 711-165-152, 1:200 Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L), Jackson ImmunoResearch 115-545-166, 1:200 Alexa Fluor 488-conjugated AffiniPure Donkey Anti-Rabbit IgG (H+L), Jackson ImmunoResearch 711-545-152, 1:200 Figure S1. ..

    Affinity Purification:

    Article Title: DDX6 interacts with DDX3X to repress translation in microRNA-mediated silencing
    Article Snippet: .. CXCR miRNA: TOP: 5’-GUU UUC ACA AAG CUA ACA CA-3’ CXCR miRNA BOTTOM: 5’-UGU UAG CUG GAG UGA AAA CUU-3’ mir-30 TOP: GCU GUA AAC AUC CUC GAC UGG AAG UU mir-30 BOTTOM: CUU UCA GUC GGA UGU UUG CAG CUU Table S3: Antibodies used in this paper DDX6 Bethyl A310-131A, rabbit anti-DDX6, 1:5000, MW 55 KDa Bethyl A310-460A, rabbit anti-DDX6, 1:5000 DDX3X Bethyl A300-474A, rabbit anti-DDX3, 1:5000, MW 75kDa Flag Sigma F3165, Anti-Flag M2 Monoclonal Antibody, 1:2000 HA Sigma H6908, rabbit anti-HA antibody, 1:2000 Roche Appplied Science, 11 583 816 001, Mouse, 1:2000 V5 AbD Serotec Clone SV5-PK1 mouse, 1:2000 Myc DSHB, 9E10 mouse antibody 1:20 GFP Invitrogen, A-11122, rabbit antibody, 1:1000 Bim Enzo Life Sciences, ADI-AAP-330, rabbit anti-Bim/BOD polyclonal Ab, 1:1000, MW 23, 16, 13 kDa Bim Cell Signaling 2933, rabbit mAb (C34C5), 1;1000, MW 23, 16, 13 kDa 4E-T Fortis, A300-706A, rabbit anti-4E-T/eIF4E-T antibody affinity purified, 1:1000, 110 KDa His Novagen 70796-4, His Tag Monoclonal antibody, 1:4000 GST Proteintech, 66001-1-lg, Mouse antibody, 1:50000, MW 26kDa Caspase 2 Cell Signaling #2224, mouse antibody, 1:1000, MW 12, 14, 48kDa GAPDH GAPDH (6C5) antibody Santa Cruz Biotechnology Cat# sc-32233, RRID:AB_627679 Dcp2 Bethyl A302-597A, Rabbit anti-Dcp2, 1:2000, MW about 55kDa Puromycin Mouse Anti-Puromycin antibody (3RH11), KeraFAST, 1:2000 eIF3A Cell Signaling 3411, anti-eIF3A (D51F4) XP TM Rabbit mAb, 1:1000, MW 166 kDa RPL7 Bethyl, A300-740A, MW 29kDa Tubulin Beta III isoform Millipore MAB1637, Mouse, 1:2000, MW 55 kDa Flag Anti-FLAG M2 Affinity Gel, Sigma, A2220 Total protein quantification LI-COR P/N 926-11010, Revert 700 Total protein Stain Kit HRP-conjugated secondary antibody Goat anti-Mouse IgG(H/L):HRP, BioRad 5178-2504, 1:100-1:5000 Goat anti-rabbit IgG(H/L):HRP, BioRad, 5196-2504 , 1:1000-1:5000 DyLight 800 secondary antibody Invitrogen SA5-35571, goat anti-Rabbit IgG DyLight 800 FITC- or Cy3 conjugated secondary antibody CyTM3-conjugated AffiniPure Donkey Anti-Rabbit IgG (H+L), Jackson ImmunoResearch, 711-165-152, 1:200 Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L), Jackson ImmunoResearch 115-545-166, 1:200 Alexa Fluor 488-conjugated AffiniPure Donkey Anti-Rabbit IgG (H+L), Jackson ImmunoResearch 711-545-152, 1:200 Figure S1. ..

    Staining:

    Article Title: DDX6 interacts with DDX3X to repress translation in microRNA-mediated silencing
    Article Snippet: .. CXCR miRNA: TOP: 5’-GUU UUC ACA AAG CUA ACA CA-3’ CXCR miRNA BOTTOM: 5’-UGU UAG CUG GAG UGA AAA CUU-3’ mir-30 TOP: GCU GUA AAC AUC CUC GAC UGG AAG UU mir-30 BOTTOM: CUU UCA GUC GGA UGU UUG CAG CUU Table S3: Antibodies used in this paper DDX6 Bethyl A310-131A, rabbit anti-DDX6, 1:5000, MW 55 KDa Bethyl A310-460A, rabbit anti-DDX6, 1:5000 DDX3X Bethyl A300-474A, rabbit anti-DDX3, 1:5000, MW 75kDa Flag Sigma F3165, Anti-Flag M2 Monoclonal Antibody, 1:2000 HA Sigma H6908, rabbit anti-HA antibody, 1:2000 Roche Appplied Science, 11 583 816 001, Mouse, 1:2000 V5 AbD Serotec Clone SV5-PK1 mouse, 1:2000 Myc DSHB, 9E10 mouse antibody 1:20 GFP Invitrogen, A-11122, rabbit antibody, 1:1000 Bim Enzo Life Sciences, ADI-AAP-330, rabbit anti-Bim/BOD polyclonal Ab, 1:1000, MW 23, 16, 13 kDa Bim Cell Signaling 2933, rabbit mAb (C34C5), 1;1000, MW 23, 16, 13 kDa 4E-T Fortis, A300-706A, rabbit anti-4E-T/eIF4E-T antibody affinity purified, 1:1000, 110 KDa His Novagen 70796-4, His Tag Monoclonal antibody, 1:4000 GST Proteintech, 66001-1-lg, Mouse antibody, 1:50000, MW 26kDa Caspase 2 Cell Signaling #2224, mouse antibody, 1:1000, MW 12, 14, 48kDa GAPDH GAPDH (6C5) antibody Santa Cruz Biotechnology Cat# sc-32233, RRID:AB_627679 Dcp2 Bethyl A302-597A, Rabbit anti-Dcp2, 1:2000, MW about 55kDa Puromycin Mouse Anti-Puromycin antibody (3RH11), KeraFAST, 1:2000 eIF3A Cell Signaling 3411, anti-eIF3A (D51F4) XP TM Rabbit mAb, 1:1000, MW 166 kDa RPL7 Bethyl, A300-740A, MW 29kDa Tubulin Beta III isoform Millipore MAB1637, Mouse, 1:2000, MW 55 kDa Flag Anti-FLAG M2 Affinity Gel, Sigma, A2220 Total protein quantification LI-COR P/N 926-11010, Revert 700 Total protein Stain Kit HRP-conjugated secondary antibody Goat anti-Mouse IgG(H/L):HRP, BioRad 5178-2504, 1:100-1:5000 Goat anti-rabbit IgG(H/L):HRP, BioRad, 5196-2504 , 1:1000-1:5000 DyLight 800 secondary antibody Invitrogen SA5-35571, goat anti-Rabbit IgG DyLight 800 FITC- or Cy3 conjugated secondary antibody CyTM3-conjugated AffiniPure Donkey Anti-Rabbit IgG (H+L), Jackson ImmunoResearch, 711-165-152, 1:200 Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L), Jackson ImmunoResearch 115-545-166, 1:200 Alexa Fluor 488-conjugated AffiniPure Donkey Anti-Rabbit IgG (H+L), Jackson ImmunoResearch 711-545-152, 1:200 Figure S1. ..



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